Plant Tissue Culture, Development, and Biotechnology: 1st Edition (Paperback) book cover

Plant Tissue Culture, Development, and Biotechnology

1st Edition

Edited by Robert N. Trigiano, Dennis J. Gray

CRC Press

608 pages | 192 B/W Illus.

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Paperback: 9781420083262
pub: 2010-11-19
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Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnology.

Addressing a wide variety of related topics, Plant Tissue Culture, Development, and Biotechnology gives the practical and technical knowledge needed to train the next generation of plant scientists regardless of their ultimate specialization. With the detailed perspectives and hands-on training signature to the authors’ previous bestselling books, Plant Development and Biotechnology and Plant Tissue Culture Concepts and Laboratory Exercises, this book discusses relevant concepts supported by demonstrative laboratory experiments. It provides critical thinking questions, concept boxes highlighting important ideas, and procedure boxes giving precise instruction for experiments, including step-by-step procedures, such as the proper microscope use with digital photography, along with anticipated results, and a list of materials needed to perform them.

Integrating traditional plant sciences with recent advances in plant tissue culture, development, and biotechnology, chapters address germplasm preservation, plant growth regulators, embryo rescue, micropropagation of roses, haploid cultures, and transformation of meristems. Going beyond the scope of a simple laboratory manual, this book also considers special topics such as copyrights, patents, legalities, trade secrets, and the business of biotechnology.

Focusing on plant culture development and its applications in biotechnology across a myriad of plant science specialties, this text uses a broad range of species and practical laboratory exercises to make it useful for anyone engaged in the plant sciences.


The Editors are to be complimented on bringing together the views of so many of the World's current experts in this field. … The Editors tried to assign each Chapter to one or a group of experts in the field. This has ensured broad/in-depth coverage of the various topics selected. Section I … outlines what they wished to achieve with this valuable contribution. … [Section II] is invaluable for those students and/or researchers wishing to enter this field. … The use of protoplasts, haploid culture, embryo rescue, somaclonal variation, cryopreservation, gene expression regulation and the production of natural products are expertly covered in [Section IV]. These Chapters form an integrated unit indicating that much time and effort has gone into its compilation. … I found this Book very comprehensive with much to offer the students, teachers and researchers. It is a valuable contribution to possess and it will remain of value for a very long time. It would be advisable for all Libraries to have copies on their shelves.

—J. Van Staden, Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, South Africa, in South African Journal of Botany, 2011, Vol. 77, Issue 3

… some interesting and well-referenced articles are included … . The book also comes with a DVD of Powerpoint presentations and 'movie' presentations of lab work which will be useful to many readers. There are some good mini-reviews with the chapter on molecular approaches a particularly useful overview. There are also some excellent introductory chapters (notably a chapter on the proper use of microscopes) which would be of great value to students and teachers. … The final chapter on The Business of Biotechnology is … a useful addition. This is an extensive volume … as a general work covering plant biotechnology would provide useful information to plant scientists.

—S. Millam, in Experimental Agriculture, 2011, Vol. 47, Issue 3

Table of Contents


Introduction to plant tissue culture, development and biotechnology, D.J. Gray and R.N. Trigiano


Getting started with tissue culture — media preparation, sterile technique and laboratory equipment, C.A. Beyl

Laboratory Exercise: Nutrition of cell and organ cultures, J. Capponetti and R.N. Trigiano

Plant growth regulators in plant tissue culture and development, C.A. Beyl

Elements of in vitro research, M.E. Compton

Concept/Laboratory Exercise: Proper use of microscopes, D.T. Webb

Concept/Laboratory Exercise:Plant histological techniques, R.N. Trigiano, D.J. Gray, K.R. Malueg, K.A. Pickens, Z.-M. Cheng, and E.T. Graham

A brief introduction to plant anatomy and morphology, R.N. Trigiano, J.A. Franklin and D.J. Gray

Seed development and germination, F. Chen, R.C. Martin, S. Song and H. Nonogaki

Concept/Labortory Exercise: Molecular tools for studying plant genetic diversity, T. Rinehart, X. Wang, R.N. Trigiano, N.R. Rowland and R.E. DeVries

Molecular approaches to the study of plant development, A. von Arnim and B.-H. Kim


Propagation from meristematic tissue -- shoot culture, M.E. Kane

Laboratory Exercise: Micropropagation of Sygonium by shoot culture, M.E. Kane

Laboratory Exercise:Micropropagation and in vitro flowering of rose, M.E. Kane, T. Johnson and P. Kauth

Laboratory Exercise: Micropropagation of potato by node culture and microtuber production, M.E. Kane

Commercial laboratory production, G.R.L. Suttle

Detection and elimination of microbial endophytes and prevention of contamination in plant tissue cultures, A.C. Cassells

Laboratory Exercise: Culture indexing for bacterial and fungal contaminants, M.E. Kane, P. Kauth and T. Johnson

Propagation from nonmeristematic tissues -- Organogenesis, R. Geneve

Developing a molecular understanding of in vitro and in planta shoot organogenesis, L. Meng, S. Zhang and P.G. Lemaux

Laboratory Exercise: Direct shoot organogenesis from chrysanthemum and african violet leaves, R.N. Trigiano, L.M. Vito, M.T. Windham, S. Boggus, and D. Hadziabdic

Propagation from nonmeristematic tissues - nonzygotic embryogenesis, D.J. Gray

Developmental and molecular aspects of nonzygotic (somatic) embryogenesis, X. Yang and X. Zhang

Laboratory Exercise: Embryogenic callus and suspension cultures from leaves of orchardgrass, D.J. Gray, R.N. Trigiano and B.V. Conger

Laboratory Exercise: Direct non-zygotic embryogenesis from leaves of cineraria, R.N. Trigiano, M.C. Scott, and K.R. Malueg


Protoplasts – an increasingly valuable tool in plant research, J.W. Grosser and A.A. Omar

Laboratory Exercise: Demonstration of principles of protoplast isolation using chrysanthemum and orchardgrass leaves, R.N. Trigiano

Laboratory Exercise:Isolation, culture and fusion of tobacco and potato protoplasts, R.E. Veilleux and M.E. Compton

Haploid cultures, D. Hadziabdic, P.A. Wadl and S.M. Reed

Laboratory Exercise: Haploid plants from tobacco and potato anthers, P.A. Wadl, D. Hadziabdic and S.M. Reed

Concept/Laboratory Exercise: Embryo rescue, T. Eeckhaut , K. Van Laere and J. Van Huylenbroeck

Promoters and gene expression regulation, Z.T. Li and D.J. Gray

Genetic engineering technologies, Z.T. Li, S.A. Dhekney and D.J. Gray

Concept/Laboratory Exercise: Transformation of plant meristems, J.H. Gould

Laboratory Exercise: Genetic transformation of chysanthemum and tobacco using Agrobacterium tumefaciens, M.M. Young, L. Padegimas, N.A. Reichert and R.N. Trigiano

Laboratory Exercise: Genetic transformation of tobacco and production of transgenic plants, S.A. Dhekney, Z.T. Li and D.J. Gray

Genetically modified plant controversies: Sensational headlines versus pragmatic research, H.A. Richards, L.C. Hudson, M.D. Halfhill and C.N. Stewart, Jr.

Cryopreservation of plant cells, tissues and organs, B.M. Reed, M.N. Normah and S.V. Kushnarenko

Laboratory Exercise: Vitrification as a method to cryopreserve shoot tips, S.V. Kushnarenko, B.M. Reed and M.N. Normah

Laboratory Exercise:Cryopreservation of orthodox and recalcitrant seed, M.N. Normah, W.K. Choo, S.V. Kushnarenko and B. Reed

Plant Biotechnology for the Production of Natural Products, A. Kirakosyan, E. M. Seymour and P. Kaufman

Laboratory Exercise: Pigment production in Ajuga cell culture, M.A. Lila and R.B. Rogers

Variations in tissue culture, R.M. Skirvin and M. Norton


Biotechnology entrepreneurship in the 21st century: From bench to bag, D.W. Altman

Intellectual property protection for plants, C. Eisenshank


Subject Categories

BISAC Subject Codes/Headings:
MEDICAL / Biotechnology
SCIENCE / Life Sciences / Biology / General
SCIENCE / Life Sciences / Botany
SCIENCE / Life Sciences / Biology / Molecular Biology
SCIENCE / Physics
TECHNOLOGY & ENGINEERING / Agriculture / General