Plant Tissue Culture Concepts and Laboratory Exercises: 2nd Edition (Paperback) book cover

Plant Tissue Culture Concepts and Laboratory Exercises

2nd Edition

Edited by Robert N. Trigiano, Dennis J. Gray

CRC Press

472 pages

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pub: 1999-11-10
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Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research.

The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field. Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. New topic discussions and laboratory exercises in the Second Edition include "Micropropagation of Dieffenbachia," "Micropropagation and in vitro flowering of rose," "Propagation from nonmeristematic tissue-organogenesis," "Variation in culture" and "Tissue culture of ferns."

It is the book's extensive laboratory exercises that provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed. What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, is a veritable harvest of information for the continued study and research in plant tissue culture science.


"Instructors and students alike will be delighted by the rich array of responsive plant species and the depth of choice among laboratory exercises that are available in Plant Tissue Culture Concepts and Laboratory Exercises, 2nd edn, edited by Robert N. Trigiano and Dennis J. Gray… The book is refreshingly readable, and the sequence of laboratory exercises leads the reader/student to develop increasing skills and knowledge of the subject matter… The range of subject matter included in the book is extensive… The written protocols do provide timely reminders regarding the benefits of careful labeling of explants and the identities of species/varieties… Laboratory protocols are very complete …Certainly this book will be of practical value in planning and teaching a successful course in plant tissue culture, but it also provides a wealth of general information and will serve as a useful reference for many protocols needed in the daily operations of tissue culture research laboratories. It will be a welcome addition to the library of everyone interested in the subject…"

- John Hubstenberger, New Mexico State University, in In Vitro Cellular Developmental Biology, Nov.- Dec. 2000

"The appeal of this practical manual is that it contains very detailed information on how to establish a tissue culture laboratory, from provision of lists of equipment and chemicals and their suppliers, to procedures and protocols for preparing media and carrying out experiments…I would recommend this book highly for teachers and researchers in plant tissue culture as a valuable resource for practical plant tissue culture."

-Plant Cell, Tissue and Organ Culture, Vol. 65, 173-174, 2001

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Table of Contents

(Note: Topic chapters appear below in bold upper and lower case letters; Laboratory chapters appear below in nonbold upper and lower case letters.)


Introduction of plant tissue and cell culture


History of plant tissue and cell culture


Getting started with tissue culture-Media preparation, sterile technique and laboratory equipment

Nutrition of callus cultures

Histological techniques

Microscopy and photography

Statistical analysis of plant tissue culture data


Propagation from preexisting meristems

Micropropagation of Syngonium by shoot culture

Micropropagation of Dieffenbachia

Micropropagation of potato by node culture and microtuber production

Micropropagation of lilac

Micropropagation and in vitro flowering of rose

Propagation from nonmeristematic tissues- organogenesis

Direct shoot organogenesis from leaf explants of chrysanthemum

Shoot organogenesis of watermelon

Direct and indirect shoot organogenesis from leaves of Torenia fournieri

Shoot organogenesis from petunia leaves

Propagation from nonmeristematic tissues- Nonzygotic embryogenesis

Embryogenic callus and suspension cultures from leaves of orchardgrass

Somatic embryogenesis from seeds of melon

Somatic embryogenesis from mature peanut seed

Direct somatic embryogenesis from leaves and flower receptacles of cineraria

Somatic embryogenesis from immature seeds of yellow poplar

Introduction of somatic embryogenesis in conifers


Use of protoplasts for plant improvement

Isolation, culture and fusion of tobacco and potato protoplasts

Isolation of protoplasts from leaves of chrysanthemum and orchardgrass

Haploid cultures

Production of haploid tobacco plants using another culture


Transformation of tobacco and carrot using Agrobacterium tumefaciens and expression of the ß-glucuronidase (GUS) reporter gene

Transformation of chrysanthemum leaf explants using Agrobacterium tumefaciens

Construction and use of a simple gene gun for particle bombardment

Germplasm preservation

Vitrification as a method to cryopreserve shoot tips

Secondary product expression in vitro

Pigment production in Ajuga cell culture

In vitro plant pathology


Variation in culture

Tissue culture of ferns

Commercial laboratory production

Indexing for plant pathogens

Culture indexing for bacterial and fungal contaminants



Subject Categories

BISAC Subject Codes/Headings:
SCIENCE / Life Sciences / Botany
SCIENCE / Life Sciences / General
TECHNOLOGY & ENGINEERING / Agriculture / General