1st Edition

The Laboratory Zebrafish




ISBN 9781439807439
Published December 17, 2010 by CRC Press
274 Pages 91 Color Illustrations

USD $67.95

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Book Description

Like other books in the Laboratory Animal Pocket Reference Series, this guide covers all aspects pertaining to the use of these organisms including their basic biology, humane care and management, husbandry, life support systems, regulatory compliance, technical procedures, veterinary care, and water quality management.

In the relatively brief span of only a few decades, the zebrafish has gone from being mainly a hobby fish to a mainstream model animal employed by scientists to study everything from stem cells to the basis of behavioral changes induced by drug addiction. This rapid advance has been fueled largely by numerous and impressive advances in technology, along with detailed characterization of the animal on a genetic and molecular level. These developments have allowed scientists to leverage the many advantages of the zebrafish system to address many important questions in biology and human genetics and disease.

However, there are few accepted and established standards for husbandry, management, and care for the fish in laboratory settings and even fewer comprehensive and constantly reliable resources. To this end, the goal of this handbook is to provide managers, veterinarians, investigators, technicians, and regulatory personnel with a concise yet thorough reference on zebrafish biology, care, husbandry, and management.

The aim of the book is not to set standards, but rather to arm those working with the fish with scientifically grounded principles and fundamental information that can be used to design sound fish care programs. This handbook is organized into seven chapters:

Biology
Husbandry
Life Support Systems
Management
Veterinary Care
Experimental Methodology
Resources

The final chapter, Resources, provides the zebrafish user with lists of sources of additional information on the zebrafish model, as well as key references, professional organizations, and suppliers of equipment and supplies used in zebrafish husbandry and care.

Table of Contents

BIOLOGY
INTRODUCTION
NATURAL HISTORY
GENETICS
GENETIC STRAINS
NOMENCLATURE
BEHAVIOR
ANATOMY AND PHYSIOLOGY
CLINICAL CHEMISTRY AND HEMATOLOGY
HUSBANDRY
NUTRITION AND FEEDING
BREEDING
LARVICULTURE
HOUSING DENSITY
GENETIC MANAGEMENT
IDENTIFICATION AND RECORD KEEPING
TRANSPORTATION
LIFE SUPPORT
FACILITY DESIGN
HOUSING SYSTEMS
WATER SOURCE
WATER QUALITY MANAGEMENT
EFFLUENT MANAGEMENT
MANAGEMENT
REGULATORY AGENCIES AND COMPLIANCE
INSTITUTIONAL ANIMAL CARE AND USE COMMITTEES
OCCUPATIONAL HEALTH AND ZOONOTIC DISEASES
DISPOSITION OF FISH
VETERINARY CARE
BASIC BIOLOGIC PARAMETERS
ANIMAL WELFARE INDICATORS FOR FISH
STRESS RESPONSE IN FISH
CLINICAL CASE MANAGEMENT AND SAMPLE COLLECTION
COMMON DISEASES OF THE LABORATORY ZEBRAFISH
PREVENTIVE MEDICINE
HEALTH SURVEILLANCE AND MONITORING
ANESTHESIA AND EUTHANASIA
EXPERIMENTAL METHODOLOGY
RESTRAINT
SAMPLING METHODS
COMPOUND ADMINISTRATION
SURGICAL PROCEDURES
RESOURCES
ORGANIZATIONS
PUBLICATIONS
ELECTRONIC RESOURCES
EQUIPMENT AND SUPPLIES
APPENDIX 1.
SAMPLE ARTEMIA DECAPSULATION SOP
APPENDIX 2.
SAMPLE ZEBRAFISH FACILITY CHECKSHEET
BIBLIOGRAPHY
INDEX

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