The Metabolic Pathway Engineering Handbook : Fundamentals book cover
1st Edition

The Metabolic Pathway Engineering Handbook

Edited By

Christina Smolke

ISBN 9781439802960
Published July 28, 2009 by CRC Press
678 Pages 4 Color & 125 B/W Illustrations

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Book Description

This first volume of the Metabolic Pathway Engineering Handbook provides an overview of metabolic pathway engineering with a look towards the future. It discusses cellular metabolism, including transport processes inside the cell and energy generating reactions, as well as rare metabolic conversions. This volume also explores balances and reaction models, the regulation of metabolic pathways, and genome scale and multiscale modeling tools. It also covers developing appropriate hosts for metabolic engineering including the use of Escherichia coli, yeast, Bacillus Subtilis, Streptomyces, filamentous fungi, and mammalian cells using cell culture. Christine Smolke, who recently developed a novel way to churn out large quantities of drugs from genetically modified brewer’s yeast, is regarded as one of the most brilliant new minds in biomedical engineering. In this handbook, she brings together pioneering scientists from dozens of disciplines to provide a complete record of accomplishment in metabolic pathway engineering. With a wealth of cutting edge research and analysis, this work also serves as an invaluable resource for those seeking to add their own contributions. Organized by topic, this 3000 page reference is available as two volumes that can be purchased individually or as a set. For information on the complete handbook visit Cat No. 3923

Table of Contents

Volume I

Introduction and Preface to Handbook, Christina D. Smolke

Progression of biological synthesis methods towards commercial relevance

The field of metabolic engineering

An overview of the metabolic pathway engineering handbook

Metabolic engineering: looking towards the future

Section I. Cellular Metabolism, Vincent Martin

Transport processes inside the cell

Energy generating reactions

Biosynthesis of important building blocks

Polymerization of building blocks to macromolecules

Rare metabolic conversions – harvesting diversity through nature

Section II. Balances and Reaction Models, Walter van Gulik

Growth nutrients and diversity

Mass balances, rates, and experiments

Data reconciliation and error detection

Black box models for growth and product formation

Metabolic models for growth and product formation

A thermodynamic description of microbial growth

Section III. Regulation of Metabolic Pathways, James Liao

Regulation of specific pathways

Regulating of secondary metabolism

Building networks as assemblies of simpler control schemes

Section IV. Modeling Tools for Metabolic Engineering, Costas Maranas

Metabolic flux analysis

Metabolic control analysis

Structure and flux analysis of metabolic networks

Constraint-based genome-scale models of cellular metabolism

Multi-scale modeling of metabolic regulation

Validation of metabolic models

Section V. Developing Appropriate Hosts for Metabolic Engineering, Jens Nielsen

Escherichia coli as a well-developed host for metabolic engineering

Metabolic engineering in yeast

Metabolic engineering in Bacillus subtilis

Metabolic engineering in Streptomyces

Metabolic engineering in filamentous fungi

Metabolic engineering in mammalian cells using cell culture

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