1st Edition

The Metabolic Pathway Engineering Handbook Fundamentals

Edited By Christina Smolke Copyright 2010
    678 Pages 4 Color & 125 B/W Illustrations
    by CRC Press

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    This first volume of the Metabolic Pathway Engineering Handbook provides an overview of metabolic pathway engineering with a look towards the future. It discusses cellular metabolism, including transport processes inside the cell and energy generating reactions, as well as rare metabolic conversions. This volume also explores balances and reaction models, the regulation of metabolic pathways, and genome scale and multiscale modeling tools. It also covers developing appropriate hosts for metabolic engineering including the use of Escherichia coli, yeast, Bacillus Subtilis, Streptomyces, filamentous fungi, and mammalian cells using cell culture. Christine Smolke, who recently developed a novel way to churn out large quantities of drugs from genetically modified brewer’s yeast, is regarded as one of the most brilliant new minds in biomedical engineering. In this handbook, she brings together pioneering scientists from dozens of disciplines to provide a complete record of accomplishment in metabolic pathway engineering. With a wealth of cutting edge research and analysis, this work also serves as an invaluable resource for those seeking to add their own contributions. Organized by topic, this 3000 page reference is available as two volumes that can be purchased individually or as a set. For information on the complete handbook visit Cat No. 3923

    Volume I

    Introduction and Preface to Handbook, Christina D. Smolke

    Progression of biological synthesis methods towards commercial relevance

    The field of metabolic engineering

    An overview of the metabolic pathway engineering handbook

    Metabolic engineering: looking towards the future

    Section I. Cellular Metabolism, Vincent Martin

    Transport processes inside the cell

    Energy generating reactions

    Biosynthesis of important building blocks

    Polymerization of building blocks to macromolecules

    Rare metabolic conversions – harvesting diversity through nature

    Section II. Balances and Reaction Models, Walter van Gulik

    Growth nutrients and diversity

    Mass balances, rates, and experiments

    Data reconciliation and error detection

    Black box models for growth and product formation

    Metabolic models for growth and product formation

    A thermodynamic description of microbial growth

    Section III. Regulation of Metabolic Pathways, James Liao

    Regulation of specific pathways

    Regulating of secondary metabolism

    Building networks as assemblies of simpler control schemes

    Section IV. Modeling Tools for Metabolic Engineering, Costas Maranas

    Metabolic flux analysis

    Metabolic control analysis

    Structure and flux analysis of metabolic networks

    Constraint-based genome-scale models of cellular metabolism

    Multi-scale modeling of metabolic regulation

    Validation of metabolic models

    Section V. Developing Appropriate Hosts for Metabolic Engineering, Jens Nielsen

    Escherichia coli as a well-developed host for metabolic engineering

    Metabolic engineering in yeast

    Metabolic engineering in Bacillus subtilis

    Metabolic engineering in Streptomyces

    Metabolic engineering in filamentous fungi

    Metabolic engineering in mammalian cells using cell culture


    Christina Smolke