Biophotonics involves understanding how light interacts with biological matter, from molecules and cells, to tissues and even whole organisms. Light can be used to probe biomolecular events, such as gene expression and protein–protein interaction, with impressively high sensitivity and specificity. The spatial and temporal distribution of biochemical constituents can also be visualized with light and, thus, the corresponding physiological dynamics in living cells, tissues, and organisms in real time. Light can also be used to alter the properties and behaviors of biological matter, such as to damage cancerous cells by laser surgery or therapy, and manipulate the neuronal signaling in a brain network. Fueled by the innovations in photonic technologies in the past half century, biophotonics continues to play a ubiquitous role in revolutionizing basic life science studies as well as biomedical diagnostics and therapies.
Advancements in biophotonics in the past few decades can be seen not only in biochemistry and cell/molecular biology, but also in numerous preclinical applications. Researchers around the world are searching for ways to bring biophotonic technologies into real clinical practices, particularly cellular and molecular optical imaging. Meanwhile, emerging technologies, such as laser nanosurgery and nanoplasmonics, have created new insights for understanding, monitoring, and even curing diseases on a molecular basis.
This book presents the essential basics of optics and biophotonics to newcomers (senior undergraduates or postgraduate researchers) who are interested in this multidisciplinary research field. With stellar contributions from leading experts, the book highlights the major advancements in preclinical diagnostics using optical microscopy and spectroscopy, including multiphoton microscopy, super-resolution microscopy, and endomicroscopy. It also introduces a number of emerging techniques and toolsets for biophotonics applications, such as nanoplasmonics, microresonators for molecular detection, and subcellular optical nanosurgery.
Table of Contents
Essential Basics of Photonics
Essential Basics of Light-Matter Interaction
Optical Coherence Tomography
Optical Scanning Holography
Calcium Imaging of Neuronal Activity
Coherent Nonlinear Microscopy
Raman Spectroscopy of Single Cells
Subcellular Optical Nanosurgery
Label-Free Detection of Nanoscale Objects Using Resonance Phenomena
Optical Transfection of Mammalian Cells
Kevin Tsia received his B.E. and M.Phil. in electronic and computer engineering from the Hong Kong University of Science and Technology, Hong Kong, in 2003 and 2005, respectively. He obtained his Ph.D. from the Electrical Engineering Department at the University of California, Los Angeles (UCLA), in 2009. He is currently an assistant professor in the Department of Electrical and Electronic Engineering, and the Medical Engineering Program, at the University of Hong Kong.
Prof. Tsia’s research interests cover a broad range of topics, including ultrafast real-time spectroscopy and microscopy for biomedical applications, such as imaging flow cytometry and endoscopic laser microsurgery. His research accomplishments, such as energy harvesting in silicon photonics and the world’s fastest barcode reader and optical imaging system, have attracted worldwide press coverage and have been featured in many prestigious science and technology review magazines such as MIT Technology Review, EE Times, and Science News. Prof. Tsia was the recipient of a fellowship by the California Nanosystems Institute (CNSI) from 2005 to 2006. He also received the 2009 Harry M. Showman Prize from the UCLA Henry Samueli School of Engineering and Applied Science and the Early Career Award 2012–13 from the Research Grants Council (RGC) in Hong Kong. He is the author or coauthor of over 90 journal articles, conference papers, and book chapters and holds 2 US patents on ultrafast optical imaging technologies.