1st Edition

Functional Lipidomics

Edited By Li Feng, Glenn D. Prestwich Copyright 2006
    352 Pages 111 B/W Illustrations
    by CRC Press

    Lipids play an essential role in cell signaling and subcellular structure. Systematic analysis of the total lipid structure of a cell or organism, the lipodome, can reveal novel avenues of therapeutic intervention and diagnosis. This analysis is best modeled after the lessons learned from proteomics. With contributions from pioneering researchers from the US, Europe, Canada, Japan, and China, Functional Lipidomics introduces lipidomics, explores the current state of the field, and provides a comprehensive background for future groundbreaking work in this burgeoning field.

    The first book dedicated to the topic, Functional Lipidomics first defines and introduces the field before delving deeply into the methods, processes, analyses, and research aspects. The book uncovers the biosynthesis, metabolism, and function of fatty acids, acylglyerols, glycerophospholipids, sphingolipids, terpenes, and lipids that complex with proteins. Taking both academic and industrial perspectives, the contributors elaborate upon cutting-edge approaches to lipid analysis, diagnostic approaches using lipid and lipid-binding protein patterns, and the therapeutic significance of targeting proteins involved with the lipid signaling pathway.

    Functional Lipidomics elucidates state-of-the-art strategies and techniques that enable us to understand biochemical patterns related to disease states, develop new targets for drug design, and identify important markers of a developmental stage, pathological condition, or disease treatment.

    The LIPID MAPS Approach to Lipidomics
    Edward A. Dennis, H. Alex Brown, Raymond A. Deems, Christopher K. Glass, Alfred H. Merrill, Jr., Robert C. Murphy, Christian R.H. Raetz, Walter Shaw, Shankar Subramaniam, David W. Russell, Michael S. VanNieuwenhze, Stephen H. White, Joseph L. Witztum, and John Wooley
    LC/MS Methodology in Lipid Analysis and Structural Characterization of Novel Lipid Species
    Robert C. Murphy, Jessica Krank, and Robert M. Barkley
    Functional Plasticity of Lipid Mediators: The Example of Endocannabinoids
    Luciano De Petrocellis, Mario van der Stelt, and Vincenzo Di Marzo
    Eicosanoid Lipidomics
    Rebecca C. Bowers-Gentry, Raymond A. Deems, Richard Harkewicz, and Edward A. Dennis
    Functional Lipidomics: Lysophosphatidic Acid as a Target for Molecular Diagnosis and Therapy of Ovarian Cancer
    Janos L. Tanyi, David Crotzer, Judith Wolf, Shuangxing Yu, Yutaka Hasegawa, John Lahad, Kwai Wa Cheng, Makiko Umezu-Goto, Glenn D. Prestwich, Andrew Morris, Robert A. Newman, Edward A. Felix, Rose Lapis, and Gordon B. Mills
    Analysis of Lysophospholipids in Human Body Fluids: Comparison of the Lysophospholipid Content in Malignant vs. Nonmalignant Diseases
    Yi Jin Xiao and Yan Xu
    Functional Lipidomics: Lessons and Examples from the Sphingolipids
    Yusuf A. Hannun
    Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Analysis of Sphingolipids
    M. Cameron Sullards and Alfred H. Merrill, Jr.
    Methods of Probing Phosphoinositides–Protein Interactions
    Li Feng, Colin Ferguson, Paul O. Neilsen, Leena Chakravarty, Piotr W. Rzepecki, and Glenn D. Prestwich
    Fishing for Pharmaceutically Relevant phosphoinositide-Binding Proteins Using Chemical Proteomics
    Christian Pasquali and Christian Rommel
    Phosphoinositide Profiling in Complex Lipid Mixtures
    Markus R. Wenk and Pietro De Camilli
    Multiplexed Lipid Arrays of Antiimmunoglobulin M–Induced Changes in the Glycerophospholipid Composition of WEHI-231 Cells
    Stephen B. Milne, Jeffrey S. Forrester, Pavlina T. Ivanova, Michelle D. Armstrong, and H. Alex Brown
    Specific Lipid Alterations in Alzheimer’s Disease and Diabetes: Shotgun Global Cellular Lipidome Analyses by Electrospray Ionization Mass Spectrometry Using Intrasource Separation
    Xianlin Han and Richard W. Gross
    High-Throughput Lipid Profiling to Identify and Characterize Genes Involved in Lipid Metabolism, Signaling, and Stress Response
    Ruth Welti, Jyoti Shah, Steven LeVine, Wynn Esch, Todd Williams, and Xuemin Wang


    Li Feng, Glenn D. Prestwich