764 pages | 141 B/W Illus.
Praise for the First Edition
“essential reading for any physical scientist who is interested in performing biological research.” ―Contemporary Physics
"an ambitious text…. Each chapter contains protocols and the conceptual reasoning behind them, which is often useful to physicists performing biological experiments for the first time." –Physics Today
This fully updated and expanded text is the best starting point for any student or researcher in the physical sciences to gain firm grounding in the techniques employed in molecular biophysics and quantitative biology. It includes brand new chapters on gene expression techniques, advanced techniques in biological light microscopy (super-resolution, two-photon, and fluorescence lifetime imaging), holography, and gold nanoparticles used in medicine. The author shares invaluable practical tips and insider’s knowledge to simplify potentially confusing techniques. The reader is guided through easy-to-follow examples carried out from start to finish with practical tips and insider’s knowledge. The emphasis is on building comfort with getting hands "wet" with basic methods and finally understanding when and how to apply or adapt them to address different questions.
Jay L. Nadeau is a scientific researcher and head of the Biomedical Engineering in Advanced Applications of Quantum, Oscillatory, and Nanotechnological Systems (BEAAQONS) lab at Caltech and was previouslyassociate professor of biomedical engineering and physics at McGill University.
"I was really very surprised how much I want to purchase this text. Our lab is engaged in research that spans multiple disciplines, from genetic engineering, biomaterials, immunotherapy, and tissue engineering. Hence the ‘tool box’ that members of my group have to develop is broad but requires detail. This text by Nadeau amazingly touches on the many if not all the techniques a modern molecular biology/bioengineering researcher needs."
—James D. Bryers, Professor of Bioengineering, University of Washington
"This book will prove to be extremely useful and motivating for any scientist, student or newcomer interested in applying concepts, methods and techniques of the physical and chemical sciences to fundamental problems in biology and medicine. It clearly enlightens the need of combining interdisciplinary approaches to tackle the main challenges in life sciences. The main strength of this book is that it provides not only some description of basic methods and techniques, but also gives access to more advanced approaches and to more sophisticated equipment. It brings physics and chemistry to biologists and physicians, as well as biology and medicine to physicists and chemists.
—Alain Arneodo, Directeur de Recherche, CNRS
Chapter 1 Introduction and Background
Chapter 2 Basic Molecular Cloning of DNA and RNA
Chapter 3 Expression of Genes in Bacteria, Yeast, and Cultured Mammalian Cells
Chapter 4 Advanced Topics in Molecular Biology
Chapter 5 Protein Expression Methods
Joshua A. Maurer
Chapter 6 Protein Crystallization
Oliver M. Baettig and Albert M. Berghuis
Chapter 7 Introduction to Biological Light Microscopy
Coauthored with Michael W. Davidson
Chapter 8 Advanced Light Microscopy Techniques
Coauthored with Lina Carlini
Chapter 9 Advanced Topics in Microscopy II: Holographic Microscopy
Coauthored with Manuel Bedrossian
Chapter 10 Quantitative Cell Culture Techniques
Chapter 11 Semiconductor Nanoparticles (Quantum Dots)
Chapter 12 Gold Nanoparticles
Edward S. Allgeyer, Gary Craig, Sanjeev Kumar Kandpal, Jeremy Grant, and Michael D. Mason
Chapter 13 Advanced Topics in Gold Nanoparticles: Biomedical Applications
Chapter 14 Surface Functionalization Techniques
Chapter 15 Electrophysiology
Coauthored with Christian A. Lindensmith and Thomas Knöpfel
Chapter 16 Spectroscopy Tools and Techniques
Chapter 17 Introduction to Nanofabrication
Appendix A: Common Solutions
Appendix B: Common Media
Appendix C: Restriction Endonucleases
Appendix D: Common Enzymes
Appendix E: Fluorescent Dyes and Quenchers
Appendix F: Fluorescent Proteins