Molecular Detection of Foodborne Pathogens  book cover
1st Edition

Molecular Detection of Foodborne Pathogens

Edited By

Dongyou Liu

ISBN 9781420076431
Published July 28, 2009 by CRC Press
905 Pages 74 B/W Illustrations

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Book Description

While the vast majority of our food supplies are nutritious and safe, foodborne pathogen-related illness still affects millions of people each year. Large outbreaks of foodborne diseases — such as the recent salmonella outbreak linked to various peanut butter products — continue to be reported with alarming frequency.

All-Encompassing Guide to Detection Techniques

One of the most effective ways to control and prevent human foodborne infections is to implement a pathogen surveillance system. Addressing the biology, epidemiology, and pathogenesis of more than 60 microorganisms, Molecular Detection of Foodborne Pathogens demonstrates how to implement a surveillance system that uses state-of-the-art molecular techniques to quickly detect and identify bacterial, fungal, viral, and parasitic pathogens concerned.

Included in each chapter:

  • Concise review of the selected pathogen in respect to its biology, epidemiology, and pathogenesis
  • Summary of molecular detection methods available
  • Description of clinical/food sample collection and preparation procedures
  • Selection of robust, effective, step-by-step detection protocols
  • Discussion of the current challenges and ongoing research needs to further extend utility and performance of molecular diagnostic methods

With proven, ready-to-use protocols (including commercial kits), this globally pertinent resource demonstrates how speedy and effective detection methods can lead to more lives saved and a consistently safe, quality, and trustworthy food supply.

Table of Contents

Molecular Detection: Principles and Methods, L. Gorski and A. Csordas


Adenoviruses, C.P. Gerba and R.A. Rodríguez

Astroviruses, E. Meleg and F. Jakab

Avian Influenza Virus, G. Cattoli and I. Monne

Hepatitis A and E Viruses, H. Ushijima, P. Khamrin, and N. Maneekarn

Noroviruses, A.C. Schultz, J. Vinjé, and B. N’ørrung

Rotaviruses, D. Liu, L.A. Hanson, and L.M. Pinchuk

Sapoviruses, G.S. Hansman

Slow Viral Diseases, T. Onodera, G. Xue, A. Sakudo, G. Zanusso, and K. Sugiura


Bacillus, N. Raddadi, A. Rizzi, L. Brusetti, S. Borin, I. Tamagnini, and D. Daffonchio

Clostridium, A. Heikinheimo, M. Lindström, D. Liu and H. Korkeala

Enterococcus, T. Semedo-Lemsaddek, R. Tenreiro, P. Lopes Alves, and M.T. Barreto Crespo

Helicobacter, N. Noguchi

Kocuria, E. Carretto and D. Barbarini

Listeria, D. Liu and H.-J. Busse

Micrococcus, F. Hilbert and H.-J. Busse

Mycobacterium, I.R. Grant and C.E.D. Rees

Staphylococcus, P. Moroni, G. Pisoni, P. Cremonesi, and B. Castiglioni

Streptococcus, M. van der Linden, R. Storm Haylett, R.R. Reinert, and L. Rink


Aeromonas, G. Naharro, J. Riaño, L. de Castro, S. Alvarez, and J.M. Luengo

Arcobacter, K. Houf

Bacteroides, R. Chaudry, A. Pandey, and N. Sharma

Brucella, S. Al Dahouk, K. Nöckler, and H. Tomaso

Burkholderia, K.H. Lynch and J.J. Dennis

Campylobacter, A. Fernández Astorga and R. Alonso

Enterobacter, A. Lehner, R. Stephan, C. Iversen, and S. Fanning

Escherichia, D.H. Shah, S. Shringi, T.E. Besser, and D.R. Call

Klebsiella, B. Meurer Moreira, M.A. Lemos Miguel, A.C. Dias de Castro, M.S. Alves, and R.C. da Silva Dias

Plesiomonas, J.A. Santos, A. Otero, and M.-L. García López

Proteus, A. Rózalski and P. Staczek

Pseudomonas, O. Zaborina and J. Alverdy

Salmonella, C. Löfström, J. Hoorfar, J. Schelin, P. Rådström, and B. Malorny

Serratia, Z.-Q. Hu, W.-H. Zhao, and Z. Hu

Shigella, B.R. Warren, K.A. Lampel, and K.R. Schneider

Vibrio, A.K. Bej

Yersinia, M. Skurnik, P. Rådström, R. Knutsson, B. Segerman, S. Hallanvuo, S.T. Lambertz, H. Korkeala, and M. Fredriksson-Ahomaa


Alternaria, D. Liu, S.B. Pruett, and C. Coyne

Aspergillus, G. Perrone, A. Gallo, and A. Susca

Candida, P.L. White, S.J. Hibbitts, M.D. Perry, and R.A. Barnes

Debaryomyces, J.J. Córdoba, M.J. Andrade, E. Bermúdez, F. Núñez, M.A. Asensio, and M. Rodríguez

Fusarium, A. Moretti and A. Susca

Penicillium, J. Dupont

Rhodotorula, D. Libkind and J.P. Sampaio

Saccharomyces, F. Rossi and S. Torriani


Acanthamoeba, H. Yera, P. Goldschmidt, C. Chaumeil, M. Cornet, and M.-L. Dardé

Cryptosporidium, U. Ryan and S.M. Cacciò

Cyclospora, D. Liu, G.T. Pharr, and F.W. Austin

Entamoeba, D. Stark and J. Ellis

Encephalitozoon and Enterocytzoon, J.J. Verweij and D. Liu

Giardia, Y. Feng and L. Xiao

Isospora, S. Jongwutiwes and C. Putaporntip

Sarcocystis, B.M. Rosenthal

Toxoplasma, C. Su and J.P. Dubey


Anisakis, S. D’Amelio, M. Busi, S. Ingrosso, L. Paggi, and E. Giuffra

Clonorchis, H. Mehlhorn, B. Müller, and J. Schmidt

Diphyllobothrium, J. Dupouy-Camet and H. Yera

Fasciola, X.-Q. Zhu, Q.-J. Zhuang, R.-Q. Lin, and W.-Y. Huang

Heterophyidae, R. Dzikowski and M.G. Levy

Metagonimus, J.-R Yu and J.-Y. Chai

Opisthorchis, P. Sithithaworn, T. Laha, and R.H. Andrews

Paragonimus, K. Narain, T. Agatsuma, and D. Blair

Taenia, A. Ito, M. Nakao, Y. Sako, K. Nakaya, T. Yanagida, and M. Okamoto

Trichinella, E. Pozio and G. La Rosa

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Dongyou Liu, Ph.D., is currently a research scientist at Human Genetic Signatures, North Ryde, NSW, Australia. Previously, he graduated in 1982 with a veterinary science degree from Hunan Agricultural University, China, and then completed his postgraduate study on immunological diagnosis of human hydatid disease in 1989 at the University of Melbourne, Australia. During the past two decades, he has worked in several research and clinical laboratories in Australia and the United States of America, with emphases on the molecular mechanisms of bacterial pathogenicity and the development of nucleic acid-based assays for species- and virulence-specific determination of microbial pathogens such as ovine footrot bacterium (Dichelobacter nodosus), dermatophyte fungi (Trichophyton, Microsporum and Epidermophyton) and listeriae (Listeria species). He is the editor of Handbook of Listeria monocytogenes and Handbook of Nucleic Acid Purification, both of which are published by CRC Press.


 … destined to be a reference text of the future.
International Food Hygiene