1st Edition

Molecular Detection of Foodborne Pathogens

Edited By Dongyou Liu Copyright 2010
    906 Pages 74 B/W Illustrations
    by CRC Press

    While the vast majority of our food supplies are nutritious and safe, foodborne pathogen-related illness still affects millions of people each year. Large outbreaks of foodborne diseases — such as the recent salmonella outbreak linked to various peanut butter products — continue to be reported with alarming frequency.

    All-Encompassing Guide to Detection Techniques

    One of the most effective ways to control and prevent human foodborne infections is to implement a pathogen surveillance system. Addressing the biology, epidemiology, and pathogenesis of more than 60 microorganisms, Molecular Detection of Foodborne Pathogens demonstrates how to implement a surveillance system that uses state-of-the-art molecular techniques to quickly detect and identify bacterial, fungal, viral, and parasitic pathogens concerned.

    Included in each chapter:

    • Concise review of the selected pathogen in respect to its biology, epidemiology, and pathogenesis
    • Summary of molecular detection methods available
    • Description of clinical/food sample collection and preparation procedures
    • Selection of robust, effective, step-by-step detection protocols
    • Discussion of the current challenges and ongoing research needs to further extend utility and performance of molecular diagnostic methods

    With proven, ready-to-use protocols (including commercial kits), this globally pertinent resource demonstrates how speedy and effective detection methods can lead to more lives saved and a consistently safe, quality, and trustworthy food supply.

    Molecular Detection: Principles and Methods, L. Gorski and A. Csordas


    Adenoviruses, C.P. Gerba and R.A. Rodríguez

    Astroviruses, E. Meleg and F. Jakab

    Avian Influenza Virus, G. Cattoli and I. Monne

    Hepatitis A and E Viruses, H. Ushijima, P. Khamrin, and N. Maneekarn

    Noroviruses, A.C. Schultz, J. Vinjé, and B. N’ørrung

    Rotaviruses, D. Liu, L.A. Hanson, and L.M. Pinchuk

    Sapoviruses, G.S. Hansman

    Slow Viral Diseases, T. Onodera, G. Xue, A. Sakudo, G. Zanusso, and K. Sugiura


    Bacillus, N. Raddadi, A. Rizzi, L. Brusetti, S. Borin, I. Tamagnini, and D. Daffonchio

    Clostridium, A. Heikinheimo, M. Lindström, D. Liu and H. Korkeala

    Enterococcus, T. Semedo-Lemsaddek, R. Tenreiro, P. Lopes Alves, and M.T. Barreto Crespo

    Helicobacter, N. Noguchi

    Kocuria, E. Carretto and D. Barbarini

    Listeria, D. Liu and H.-J. Busse

    Micrococcus, F. Hilbert and H.-J. Busse

    Mycobacterium, I.R. Grant and C.E.D. Rees

    Staphylococcus, P. Moroni, G. Pisoni, P. Cremonesi, and B. Castiglioni

    Streptococcus, M. van der Linden, R. Storm Haylett, R.R. Reinert, and L. Rink


    Aeromonas, G. Naharro, J. Riaño, L. de Castro, S. Alvarez, and J.M. Luengo

    Arcobacter, K. Houf

    Bacteroides, R. Chaudry, A. Pandey, and N. Sharma

    Brucella, S. Al Dahouk, K. Nöckler, and H. Tomaso

    Burkholderia, K.H. Lynch and J.J. Dennis

    Campylobacter, A. Fernández Astorga and R. Alonso

    Enterobacter, A. Lehner, R. Stephan, C. Iversen, and S. Fanning

    Escherichia, D.H. Shah, S. Shringi, T.E. Besser, and D.R. Call

    Klebsiella, B. Meurer Moreira, M.A. Lemos Miguel, A.C. Dias de Castro, M.S. Alves, and R.C. da Silva Dias

    Plesiomonas, J.A. Santos, A. Otero, and M.-L. García López

    Proteus, A. Rózalski and P. Staczek

    Pseudomonas, O. Zaborina and J. Alverdy

    Salmonella, C. Löfström, J. Hoorfar, J. Schelin, P. Rådström, and B. Malorny

    Serratia, Z.-Q. Hu, W.-H. Zhao, and Z. Hu

    Shigella, B.R. Warren, K.A. Lampel, and K.R. Schneider

    Vibrio, A.K. Bej

    Yersinia, M. Skurnik, P. Rådström, R. Knutsson, B. Segerman, S. Hallanvuo, S.T. Lambertz, H. Korkeala, and M. Fredriksson-Ahomaa


    Alternaria, D. Liu, S.B. Pruett, and C. Coyne

    Aspergillus, G. Perrone, A. Gallo, and A. Susca

    Candida, P.L. White, S.J. Hibbitts, M.D. Perry, and R.A. Barnes

    Debaryomyces, J.J. Córdoba, M.J. Andrade, E. Bermúdez, F. Núñez, M.A. Asensio, and M. Rodríguez

    Fusarium, A. Moretti and A. Susca

    Penicillium, J. Dupont

    Rhodotorula, D. Libkind and J.P. Sampaio

    Saccharomyces, F. Rossi and S. Torriani


    Acanthamoeba, H. Yera, P. Goldschmidt, C. Chaumeil, M. Cornet, and M.-L. Dardé

    Cryptosporidium, U. Ryan and S.M. Cacciò

    Cyclospora, D. Liu, G.T. Pharr, and F.W. Austin

    Entamoeba, D. Stark and J. Ellis

    Encephalitozoon and Enterocytzoon, J.J. Verweij and D. Liu

    Giardia, Y. Feng and L. Xiao

    Isospora, S. Jongwutiwes and C. Putaporntip

    Sarcocystis, B.M. Rosenthal

    Toxoplasma, C. Su and J.P. Dubey


    Anisakis, S. D’Amelio, M. Busi, S. Ingrosso, L. Paggi, and E. Giuffra

    Clonorchis, H. Mehlhorn, B. Müller, and J. Schmidt

    Diphyllobothrium, J. Dupouy-Camet and H. Yera

    Fasciola, X.-Q. Zhu, Q.-J. Zhuang, R.-Q. Lin, and W.-Y. Huang

    Heterophyidae, R. Dzikowski and M.G. Levy

    Metagonimus, J.-R Yu and J.-Y. Chai

    Opisthorchis, P. Sithithaworn, T. Laha, and R.H. Andrews

    Paragonimus, K. Narain, T. Agatsuma, and D. Blair

    Taenia, A. Ito, M. Nakao, Y. Sako, K. Nakaya, T. Yanagida, and M. Okamoto

    Trichinella, E. Pozio and G. La Rosa


    Dongyou Liu, Ph.D., is currently a research scientist at Human Genetic Signatures, North Ryde, NSW, Australia. Previously, he graduated in 1982 with a veterinary science degree from Hunan Agricultural University, China, and then completed his postgraduate study on immunological diagnosis of human hydatid disease in 1989 at the University of Melbourne, Australia. During the past two decades, he has worked in several research and clinical laboratories in Australia and the United States of America, with emphases on the molecular mechanisms of bacterial pathogenicity and the development of nucleic acid-based assays for species- and virulence-specific determination of microbial pathogens such as ovine footrot bacterium (Dichelobacter nodosus), dermatophyte fungi (Trichophyton, Microsporum and Epidermophyton) and listeriae (Listeria species). He is the editor of Handbook of Listeria monocytogenes and Handbook of Nucleic Acid Purification, both of which are published by CRC Press.

     … destined to be a reference text of the future.
    International Food Hygiene