This book provides a comprehensive understanding of the discovery of a new cellular structure the "porosome," which is the universal secretory machinery in cells; the protein assembly, biomineralization, and biomolecular interactions; the molecular evolution of protein structure; the use of magnetic nanoparticles for transformative application in medicine and therapy, and the new and novel imaging approach of electrical impedance spectroscopy in biology. It be used for college courses in nanomedicine, nano cell biology, advanced nanotechnology, and biotechnology at the undergraduate and graduate level.
Table of Contents
Porosomes -The Universal Secretory Portals In Cells: A Brief Essay
The Hair Cell Porosome: Molecular and Synaptic Implications
The Neuronal Porosome Complex in Mammalian Brain: A Study Using Electron Microscopy
Granule Size Distribution Suggests Mechanism: The Case for Granule Growth and Elimination as a Fusion Nano-Machine
Probing Protein Assembly, Biomineralization, and Biomolecular Interactions by Atomic Force Microscopy
High-Resolution Imaging of Amylin Aggregation and Internalization in Pancreatic Cells: Implications in Health and Diseaser
Repair of Nanodefects in a 2-Dimensional Crystal Anticoagulant Shield in the Antiphospholipid Syndrome: Novel Molecular Strategies Assessed by Atomic Force Microscopy
A novel approach to study molecular evolution: Detection of ancestral conformation hidden in present-day proteins using antibody as nanostructure probes
mRNA Nanomachines and Stress Reprogramming Following Brain Ischemia. Physical Properties and Biomedical Applications of Superparamagnetic Iron Oxide Nanoparticles
Atomic Force Microscopy Imaging of DNA Delivery Nano-systems
Impedance Spectroscopy for Characterization of Biological Functions
Bhanu P. Jena is George E. Palade University Professor and distinguished professor, Department of Physiology, Wayne State University School of Medicine, USA, and director of the NanoBioScience Institute at the university. He received his PhD from Iowa State University followed by postdoctoral studies and faculty positions at Yale University School of Medicine prior to his current position.
Douglas J. Taatjes is professor of pathology and director of the Microscopy Imaging Center in the College of Medicine, University of Vermont, USA. He received his PhD from the Biocenter, University of Basel, Switzerland, prior to his current position.
"NanoCellBiology: Multimodal Imaging in Biology and Medicine, edited by Bhanu P. Jena and Douglas J. Taatjes, is a collection of chapters that describe examples of the use of AFM, electron microscopy, photon correlation spectroscopy, confocal microscopy, fluorescence/CD spectroscopy, and other imaging approaches for revealing important structures and their function in cells. A wonderful example is the subject of the first several chapters, which describe the discovery of the porosome. Discovered in the 1990s, first in pancreatic acinar cells, the porosome is now considered a universal secretory portal in cells. The remaining chapters add to this excellent collection of studies employing high-resolution imaging to examine, for example, amylin aggregation, mRNA nanomachines, DNA delivery nanosystems, and other interesting applications of nanocellbiology."
Prof. James A. Spudich, Stanford University School of Medicine, USA
"Bhanu P. Jena is a pioneer nanocell biologist, whose seminal discovery of a new cell structure called the ‘porosome,’ has provided a molecular understanding of the fractional release of intravesicular contents from cells during secretion. In this book, co-edited by Jena and Douglas J. Taatjes, experts in the field present examples of powerful imaging modalities that have been extremely valuable in elucidating a wide range of normal cellular events, as well as in studying disease progression, detection, and treatment. Chapters in the book provide a lucid explanation of the subject matter, with ample illustrations presented for clarity. This is a timely book, filled with useful resources—a must-read for both researchers and students in cell biology, physiology, biophysics, nanobiology, and nanomedicine."
Prof. Walter F. Boron, Case Western Reserve University, USA
"NanoCellBiology: Multimodal Imaging in Biology and Medicine is a delightful book co-edited by a pioneer in the subject, Bhanu P. Jena, whose discovery of a new cellular structure, the porosome, in 1996 resulted in a paradigm shift in our understanding of cell secretion and revolutionized our understanding of the unit of life—the cell. This book, edited by Bhanu P. Jena and Douglas J. Taatjes, introduces to the reader a number of exciting subjects within the field of nanoscience and nanomedicine, and the various tools and approaches to solve them for the benefit of science and humanity. I thoroughly enjoyed reading the book and highly recommend to both students in the pure sciences and medicine."
Prof. Lloyd L. Anderson, Iowa State University, USA