This book, first of this new two-volume set, provides an informative tour of the basics of biotechnology to recent advances in biotechnology. Knowledge of new and fresh approaches is a prerequisite to solving plant biological problems, and to this end, the editors have brought together a group of contributors who address the most recent techniques and their applications in plant biotechnology. The chapters discuss some recent techniques such as TILLING (Targeting Induced Local Lesions In Genomes), advances in molecular techniques to study diversity, protein purification, and methods and analysis in protein-protein interaction detection. The volume also covers molecular markers and QTL mapping, including four chapters that deal with different molecular markers, development of mapping populations, and association mapping for dissecting the genetic basis of complex traits in plants in sufficient detail.
The knowledge of biotechnology techniques and their applications will be valuable for researchers and scientists as well as for the many students engaged in plant biotechnology studies.
Table of Contents
History of Biotechnology
Suhail Muzaffar and Bishun Deo Prasad
Scope and Importance of Plant Biotechnology in Crop Improvement
Ashish Ranjan and Devanshi Khokhani
Scope of Plant Biotechnology in the Developing Countries
Tushar Ranjan, Sangita Sahni, Bishnu Deo Prasad, Ravi Ranjan Kumar, Kumari Rajani, Vijay Kumar Jha, Vaishali Sharma, Mahesh Kumar, and Vinod Kumar
Basic Principles and Recent Advances in Anther/Pollen Culture for Crop Improvement
Govinal Badiger Bhaskara
Embryo Culture and Endosperm Culture
Manoj Kundu, Jayesh Pathak, and Sangita Sahni
Tushar Ranjan, Bishnu Deo Prasad, Sunita Kumari, Ram Balak Prasad Nirala, Ravi Ranjan Kumar, Vijay Kumar Jha, Vaishali Sharma, Md Shamim, and Anand Kumar
Protoplast Isolation and Fusion
Uday Sajja, Tushar Ranjan, and Bishnu Deo Prasad
Ashutosh Pathak and Aruna Joshi
Somaclonal Variation: A Tissue Culture Approach to Crop Improvement
Kumari Rajani, Ravi Ranjan Kumar, Tushar Ranjan, Ganesh Patil, and Anand Kumar
Shiv Shankar, Imran Uddin, and Seyedeh Fatemeh Afzali
Ligation of Insert DNA in to Cloning Vector
Tushar Ranjan, Pankaj Kumar, Bishnu Deo Prasad, Sangita Sahni, Vaishali Sharma, Sonam Kumari, Ravi Ranjan Kumar, Mahesh Kumar, Vijay Kumar Jha, and Prasant Kumar
Prasant Kumar, Mitesh Dwivedi, ChandraPrakash, Sangita Sahni, and Bishun Deo Prasad
Advances in PCR Technology and RNA Interference
TILLING: Genome Poking with Diligences and Constraints
G. Thapa and J. G. Hehir
Advances in Molecular Techniques to Study Diversity
Prasant Kumar, Mitesh Dwivedi, Mitesh B. Patel, Chandra Prakash, and Bishun Deo Prasad
Protein Purification: Science and Technology
Ganesh Patil, Ravi Ranjan Kumar, Tushar Ranjan, and Kumari Rajani
Protein-Protein Interaction Detection: Methods and Analysis
Vaishali Sharma, Tushar Ranjan, Pankaj Kumar, Awadhesh Kumar Pal, Vijay Kumar Jha, Sangita Sahni, and Bishun Deo Prasad
Molecular Markers in Plant Biotechnology
Gaurav V. Sanghvi and Gaurav S. Dave
Development of Mapping Populations
Anand Kumar, Tushar Ranjan, Ravi Ranjan Kumar, Kumari Rajani, and Chandan Kishore
Principles and Practices of Mapping QTLs in Plants
Sunayana Rathi, Akhil Ranjan Baruah, Surojit Sen, and Samindra Baishya
Association Mapping: A Tool for Dissecting the Genetic Basis of Complex Traits in Plants
Sweta Sinha, Amarendra Kumar, Renu Kushwah, and Ravi Ranjan Kumar
Bishun Deo Prasad, PhD, is an Assistant Professor and Scientist in the Department of Molecular Biology and Genetic Engineering at Bihar Agricultural University in Sabour, India. He has published 12 research papers in reputed peer-reviewed international journals and has also contributed to one authored book. He has written several book chapters, and has submitted 10 sequences of different isolates to the National Center for Biotechnology Information (NCBI). He is reviewer of International Journal of Agriculture Sciences and the Journal of Environmental Biology. Dr. Prasad has received several prestigious awards for his work. Dr. Prasad has been associated with biotechnological aspects of rice, Brassica napus, arabidopsis, linseed, lentil, vegetable (bitter guard and pointed guard), and horticultural (mango, litchi and banana) crops. He is also associated with host pathogen interaction studies in rice, B. napus and Arabidopsis as well as mutational breeding aspect in rice for abiotic stress tolerance.
Sangita Sahni, PhD, is a Junior Scientist and Assistant Professor in the Department of Plant Pathology at Tirhut College of Agriculture, Dholi, and Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur, Bihar, India. She has published 10 research papers in reputed peer-reviewed national and international journals and has published one authored book and several book chapters. She is an officer in charge of the Agricultural Research Information System (ARIS) Cell, Tirhut College of Agriculture, Dholi, and a member of different committees at Rajendra Agricultural University, Pusa, India. She has been an active member of the organizing committees of several national and international seminars. Dr. Sahni has been associated with molecular host pathogen interaction studies in Arabidopsis and B. napus. She is also associated with the pathological aspect of chickpea and MULLaRP (mungbean, urdbean, lentil, lathyrus, rajmash, and pea). She is actively involved in teaching graduate and postgraduate courses on plant pathology and biotechnology. In her research, she has isolated several bacterial isolates from different sources and submitted their sequences to the National Centre for Biotechnology Information (NCBI).
Prasant Kumar, PhD, is an Assistant Professor in the C. G. Bhakta Institute of Biotechnology, Department of Fundamental and Applied Science at Uka Tarsadia University, Surat, Gujarat, India. He is the author or co-author of 10 peer-reviewed journal articles and eight conference papers and newsletters. He is a reviewer and an editorial board member of several peer-reviewed journals. He has been an active member of the organizing committees of several national and international seminars and conferences. He previously was an Assistant Professor at Sarder Patel University, Anand, Gujarat, India.
Mohammed Wasim Siddiqui, PhD, is an Assistant Professor and Scientist in the Department of Food Science and Post-Harvest Technology, Bihar Agricultural University, Sabour, India, and author or co-author of 31 peer-reviewed research articles, 26 book chapters, two manuals, and 18 conference papers. He has over a dozen edited books and one authored book to his credit. Dr. Siddiqui has established the international peer-reviewed Journal of Postharvest Technology. He has received several grants and respected awards for his research work by a number of organizations. He is an active member of the organizing committees of several national and international seminars, conferences, and summits. He is one of key members in establishing the World Food Preservation Center (WFPC), LLC, USA, and is currently an active associate and supporter.