1st Edition

Standard Operational Procedures in Reproductive Medicine
Laboratory and Clinical Practice

ISBN 9781498719216
Published February 16, 2017 by CRC Press
198 Pages

USD $99.95

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Book Description

Compiled by two experts in Reproductive Medicine, with contributions from internationally respected specialists, this innovative text lets the whole team in Reproductive Medicine get literally on the same page. Taking a cook-book approach to the operational procedures in the laboratory and in the clinic, it details what needs to be prepared in advance, what needs to be prepared earlier the same day, and what steps to take before, during, and after the procedure itself. This is an essential tool for ensuring all staff - whether experienced or starters - can be confident in their tasks and are in touch with what is expected of them and their colleagues.

Table of Contents


1 The documentation system
Markus Montag and Lars Johansson
2 Culture Media Techniques
Lars Johansson
3 IVF incubator handling
Jason E Swain and Alex Lagunov
4 Preparation for follicle aspiration and isolation of cumulus-oocyte-complexes (COC)
Markus Montag
5 Semen collection and sample reception
Carin Huyser
6 Spermiogramm, Makler chamber, Neubauer chamber, CASA
Verena Nordhoff
7 Sperm viability testing
Markus Montag
8 Sperm preparation – Swim up, density gradient, migration chamber, zeta method
Thomas Ebner
9 Sperm selection using hyaluronan binding
Denny Sakkas
10 IVF - Insemination
Markus Montag
Thomas Freour
12 Oocyte in vitro maturation – Isolation of COCs and culture for maturation
Mariabeatrice Dal Canto and Giovanni Coticchio
13 Artificial oocyte activation
Markus Montag
14 Cryopreservation - Sperm TESE and MESA
Borut Kovačič
15 Vitrification – Oocytes, 2PN, Embryos, Blastocysts
Ana Cobo and Aila Coello
16 Slow freezing: principles, oocytes, 2PN, embryos, blastocysts
Etienne Van den Abbeel
17 Oocyte selection
Başak Balaban
18 Zygote selection
Martin Greuner
19 Selection and grading of embryos on day 2/3 and blastocysts on day 5/6
Thorir Hardarson and Julius Hreinsson
20 Time-lapse imaging
Cristina Hickman
21 Assisted hatching
Maria De Geyter and Christian De Geyter
22 Zona manipulation at various embryo stages: Mechanical / acidic medium
Marius Meintjes
23 Biopsy: polar body, blastomere, trophectoderm
Hakan Kadir Yelke and Semra Kahraman
24 Sampling of cells after biopsy for aCGH
Barry Behr
25 Embryo transfer – standard and ultrasound guided
Bettina Toth
26 Hygenic standards in the IVF laboratory
Vera Baukloh
27 Quality control of IVF laboratory supplies – Mouse Embryo Assay (MEA)
Dean E Morbeck
28 Troubleshooting in the IVF laboratory
David Mortimer and Sharon T Mortimer
29 Microscopy
Markus Montag
30 Fertility preservation
Markus Montag and Jana Liebenthron

31 Ultrasound evaluation in treatment management
Ippokratis Sarris and Geeta Nargund
32 Three-Dimensional Ultrasound and the Diagnosis of Uterine Anomalies
Magued Adel Aziz Mikhail, Megan Lively, Candice P. Holliday, and Botros Rizk
33 Infertility ultrasound evaluation: One Stop Procedure
Spiros A. Liatsikos and Geeta Nargund
34 Office Hysteroscopy
Linda D. Bradley
35 Hysteroscopic management of uterine septum
Omar Abuzeid and Mostafa Abuzeid
36 Hysteroscopic Lysis of Intrauterine Adhesions
Bolonduro Oluwamuyiwa and Mostafa Abuzeid
37 Hysteroscopic Management of Submucosal Fibroids
Salem Joseph and Mostafa Abuzeid
38 Saline infusion sonohysterography
Alan Bolnick and Mostafa Abuzeid
39 General Principles of Laparoscopy
Rubin Raju and Mostafa Abuzeid
40 Microlaparoscopy
Oscar D. Almeida Jr
41 Laparoscopic myomectomy and minilaparotomy: an alternative approach for myomectomy
Mostafa Abuzeid and Ahmed Abdel Aziz
42 Laparoscopic Excision of a Large Endometrioma
Rubin Raju and Mostafa Abuzeid
43 Laparoscopic management of subtle fimbrial pathology secondary to endometriosis
Omar Abuzeid and Mostafa Abuzeid
44 Laparoscopic management of mature cystic teratoma
Rahima Sanya and Mostafa Abuzeid
45 Tubal Cannulation for Proximal Tubal Disease
Botros Rizk and Josh Ekladios
46 Robotic assisted laparoscopic myomectomy
Hashem Lofty, Arsany Bassily, Lauren Mann, and Botros Rizk
47 Robotic Assisted Tubal Anastomosis After Tubal Sterilization
Hashem Lofty, Rola Turki, and Botros Rizk
48 Unexplained infertility
Hassan N. Sallam and Nooman H. Sallam
49 Male Evaluation for Infertility
Kiranpreet Khurana, Edmund Sabanegh, Jr., and Ashok Agarwal
50 Hysterosalpingography
Islam Fahmi, Mostafa Abuzeid, and Shawky ZA Badawy
51 Intrauterine insemination: clinical perspectives
Willem Ombelet
52 GnRH agonist to trigger final follicular maturation in IVF
Nayana Talukdar and Robert F. Casper
53 GnRH agonist Protocols
Justin Tan, Rabea Youcef Khoudja, Michael Doley, Michael Dahan and Seang Lin Tan
54 GnRH agonists before IVF for advanced endometriosis
Hassan N. Sallam and Nooman H. Sallam
55 Microdose GnRH Agonist Flare Protocols
Magued Adel Mikhail, Wael Salem, and Botros Rizk
56 Gonadotropin Releasing Hormone Antagonist Cycle for In Vitro Fertilization
Michael Dahan, Justin Tan, Baris Ata, Rabea Khoudja, Seang Lin Tan, and Botros Rizk
57 Minimal stimulation for IVF
Ippokratis Sarris and Geeta Nargund
58 Ovarian Hyperstimulation Syndrome
Magued Adel Mikhail, Daniel Antonious, Candice P. Holliday, and Botros Rizk
59 The Prediction of OHSS
Jan Gerris and Jana Claeys
60 Tips on technique of embryo transfer
Omar Abuzeid and Mostafa Abuzeid
61 Difficult Embryo Transfers
Gautam N. Allahbadia and Goral N. Gandhi
62 In vitro maturation of oocytes for fertility treatment and fertility preservation
Michael H. Dahan, Justin Tan, Rabea Youcef Khoudja, Andrew Mok, and Seang Lin Tan
63 Complications and outcomes of IVF
Jan Gerris
64 Obstetric complications after in-vitro fertilization
Brian Brocato and David Lewis
65 Twins pregnancy after ART
David F. Lewis and Brian Brocato
66 IVF and endometriosis
Graciela Kohls Ilgner and Juan Antonio Garcia Velasco
67 Leiomyoma of the Uterus
Shawky ZA Badawy
68 Office Management of Polycystic Ovarian Syndrome
Shawky ZA Badawy
69 Adenomyosis: Imaging and Treatment
Magued Adel Mikhail, Candice P. Holliday, and Botros Rizk
70 Ectopic Pregnancy: Evaluation and Management
James P. Schwayder

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Botros Rizk, MD, FRCOG, FRCS, HCLD, FACOG, FACS, is Professor and Head of Reproductive Endocrinology and Infertility, Medical and Scientific Director of In Vitro Fertilization and Assisted Reproduction, University of South Alabama, Mobile, Albama, USA; Distinguished Adjunct Professor of Reproductive Endocrinology and Infertility, Obstetrics and Gynecology, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia; and Distinguished Adjunct Scientific Director, IVF Michigan, Rochester Hills, Michigan, USA

Markus Montag, PhD, is Professor and CEO, ilabcomm GmbH, Sankt Augustin, NRW, Germany