Standard Operational Procedures in Reproductive Medicine: Laboratory and Clinical Practice, 1st Edition (Paperback) book cover

Standard Operational Procedures in Reproductive Medicine

Laboratory and Clinical Practice, 1st Edition

Edited by Botros Rizk, Markus Montag

CRC Press

180 pages

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pub: 2017-02-16
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Compiled by two experts in Reproductive Medicine, with contributions from internationally respected specialists, this innovative text lets the whole team in Reproductive Medicine get literally on the same page. Taking a cook-book approach to the operational procedures in the laboratory and in the clinic, it details what needs to be prepared in advance, what needs to be prepared earlier the same day, and what steps to take before, during, and after the procedure itself. This is an essential tool for ensuring all staff - whether experienced or starters - can be confident in their tasks and are in touch with what is expected of them and their colleagues.

Table of Contents


1 The documentation system

Markus Montag and Lars Johansson

2 Culture Media Techniques

Lars Johansson

3 IVF incubator handling

Jason E Swain and Alex Lagunov

4 Preparation for follicle aspiration and isolation of cumulus-oocyte-complexes (COC)

Markus Montag

5 Semen collection and sample reception

Carin Huyser

6 Spermiogramm, Makler chamber, Neubauer chamber, CASA

Verena Nordhoff

7 Sperm viability testing

Markus Montag

8 Sperm preparation – Swim up, density gradient, migration chamber, zeta method

Thomas Ebner

9 Sperm selection using hyaluronan binding

Denny Sakkas

10 IVF - Insemination

Markus Montag


Thomas Freour

12 Oocyte in vitro maturation – Isolation of COCs and culture for maturation

Mariabeatrice Dal Canto and Giovanni Coticchio

13 Artificial oocyte activation

Markus Montag

14 Cryopreservation - Sperm TESE and MESA

Borut Kovačič

15 Vitrification – Oocytes, 2PN, Embryos, Blastocysts

Ana Cobo and Aila Coello

16 Slow freezing: principles, oocytes, 2PN, embryos, blastocysts

Etienne Van den Abbeel

17 Oocyte selection

Başak Balaban

18 Zygote selection

Martin Greuner

19 Selection and grading of embryos on day 2/3 and blastocysts on day 5/6

Thorir Hardarson and Julius Hreinsson

20 Time-lapse imaging

Cristina Hickman

21 Assisted hatching

Maria De Geyter and Christian De Geyter

22 Zona manipulation at various embryo stages: Mechanical / acidic medium

Marius Meintjes

23 Biopsy: polar body, blastomere, trophectoderm

Hakan Kadir Yelke and Semra Kahraman

24 Sampling of cells after biopsy for aCGH

Barry Behr

25 Embryo transfer – standard and ultrasound guided

Bettina Toth

26 Hygenic standards in the IVF laboratory

Vera Baukloh

27 Quality control of IVF laboratory supplies – Mouse Embryo Assay (MEA)

Dean E Morbeck

28 Troubleshooting in the IVF laboratory

David Mortimer and Sharon T Mortimer

29 Microscopy

Markus Montag

30 Fertility preservation

Markus Montag and Jana Liebenthron


31 Ultrasound evaluation in treatment management

Ippokratis Sarris and Geeta Nargund

32 Three-Dimensional Ultrasound and the Diagnosis of Uterine Anomalies

Magued Adel Aziz Mikhail, Megan Lively, Candice P. Holliday, and Botros Rizk

33 Infertility ultrasound evaluation: One Stop Procedure

Spiros A. Liatsikos and Geeta Nargund

34 Office Hysteroscopy

Linda D. Bradley

35 Hysteroscopic management of uterine septum

Omar Abuzeid and Mostafa Abuzeid

36 Hysteroscopic Lysis of Intrauterine Adhesions

Bolonduro Oluwamuyiwa and Mostafa Abuzeid

37 Hysteroscopic Management of Submucosal Fibroids

Salem Joseph and Mostafa Abuzeid

38 Saline infusion sonohysterography

Alan Bolnick and Mostafa Abuzeid

39 General Principles of Laparoscopy

Rubin Raju and Mostafa Abuzeid

40 Microlaparoscopy

Oscar D. Almeida Jr

41 Laparoscopic myomectomy and minilaparotomy: an alternative approach for myomectomy

Mostafa Abuzeid and Ahmed Abdel Aziz

42 Laparoscopic Excision of a Large Endometrioma

Rubin Raju and Mostafa Abuzeid

43 Laparoscopic management of subtle fimbrial pathology secondary to endometriosis

Omar Abuzeid and Mostafa Abuzeid

44 Laparoscopic management of mature cystic teratoma

Rahima Sanya and Mostafa Abuzeid

45 Tubal Cannulation for Proximal Tubal Disease

Botros Rizk and Josh Ekladios

46 Robotic assisted laparoscopic myomectomy

Hashem Lofty, Arsany Bassily, Lauren Mann, and Botros Rizk

47 Robotic Assisted Tubal Anastomosis After Tubal Sterilization

Hashem Lofty, Rola Turki, and Botros Rizk

48 Unexplained infertility

Hassan N. Sallam and Nooman H. Sallam

49 Male Evaluation for Infertility

Kiranpreet Khurana, Edmund Sabanegh, Jr., and Ashok Agarwal

50 Hysterosalpingography

Islam Fahmi, Mostafa Abuzeid, and Shawky ZA Badawy

51 Intrauterine insemination: clinical perspectives

Willem Ombelet

52 GnRH agonist to trigger final follicular maturation in IVF

Nayana Talukdar and Robert F. Casper

53 GnRH agonist Protocols

Justin Tan, Rabea Youcef Khoudja, Michael Doley, Michael Dahan and Seang Lin Tan

54 GnRH agonists before IVF for advanced endometriosis

Hassan N. Sallam and Nooman H. Sallam

55 Microdose GnRH Agonist Flare Protocols

Magued Adel Mikhail, Wael Salem, and Botros Rizk

56 Gonadotropin Releasing Hormone Antagonist Cycle for In Vitro Fertilization

Michael Dahan, Justin Tan, Baris Ata, Rabea Khoudja, Seang Lin Tan, and Botros Rizk

57 Minimal stimulation for IVF

Ippokratis Sarris and Geeta Nargund

58 Ovarian Hyperstimulation Syndrome

Magued Adel Mikhail, Daniel Antonious, Candice P. Holliday, and Botros Rizk

59 The Prediction of OHSS

Jan Gerris and Jana Claeys

60 Tips on technique of embryo transfer

Omar Abuzeid and Mostafa Abuzeid

61 Difficult Embryo Transfers

Gautam N. Allahbadia and Goral N. Gandhi

62 In vitro maturation of oocytes for fertility treatment and fertility preservation

Michael H. Dahan, Justin Tan, Rabea Youcef Khoudja, Andrew Mok, and Seang Lin Tan

63 Complications and outcomes of IVF

Jan Gerris

64 Obstetric complications after in-vitro fertilization

Brian Brocato and David Lewis

65 Twins pregnancy after ART

David F. Lewis and Brian Brocato

66 IVF and endometriosis

Graciela Kohls Ilgner and Juan Antonio Garcia Velasco

67 Leiomyoma of the Uterus

Shawky ZA Badawy

68 Office Management of Polycystic Ovarian Syndrome

Shawky ZA Badawy

69 Adenomyosis: Imaging and Treatment

Magued Adel Mikhail, Candice P. Holliday, and Botros Rizk

70 Ectopic Pregnancy: Evaluation and Management

James P. Schwayder

About the Editors

Botros Rizk, MD, FRCOG, FRCS, HCLD, FACOG, FACS, is Professor and Head of Reproductive Endocrinology and Infertility, Medical and Scientific Director of In Vitro Fertilization and Assisted Reproduction, University of South Alabama, Mobile, Albama, USA; Distinguished Adjunct Professor of Reproductive Endocrinology and Infertility, Obstetrics and Gynecology, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia; and Distinguished Adjunct Scientific Director, IVF Michigan, Rochester Hills, Michigan, USA

Markus Montag, PhD, is Professor and CEO, ilabcomm GmbH, Sankt Augustin, NRW, Germany

About the Series

Reproductive Medicine and Assisted Reproductive Techniques Series

Learn more…

Subject Categories

BISAC Subject Codes/Headings:
MEDICAL / General
MEDICAL / Gynecology & Obstetrics
MEDICAL / Reproductive Medicine & Technology