Methods in Chemosensory Research  book cover
1st Edition

Methods in Chemosensory Research

ISBN 9780849323294
Published September 26, 2001 by CRC Press
560 Pages 80 B/W Illustrations

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Book Description

Written by world experts in the field of neuronal chemoreception, Methods in Chemosensory Research describes new molecular, electrophysiological, genetic, behavioral, psychophysical, and imaging techniques that have recently been adapted to investigate the basic neuronal mechanisms underlying chemoreception: taste, olfaction and chemical-induced irritation. This unique book includes a wide variety of new and innovative imaging methods, electrophysiological methods, methods to investigate development, and various aspects of behavioral testing.

Covering the entire spectrum of chemoreception, each section not only describes what information can be obtained by using each method, but also shows how to apply each method to obtain reliable results. The book presents a comprehensive view of how the brain uses information about chemical stimuli that interact with receptors in the nose and tongue. Methods in Chemosensory Research gives you the new tools to learn how chemicals affect activity from the genetic to the behavioral levels.

Table of Contents

Behavioral Studies
Psychophysical Measurement of Oral Chemesthesis
Behavioral Methods in Olfactory Research with Rodents

Molecular and Cell Biology of Taste and Olfaction
Recordings from Vertebrate Olfactory Receptor Neurons: From Isolated Cells to Intact Epithelial Preparations
Recordings from Olfactory Receptor Neurons in the Rat in Vivo
Voltage-Sensitive and Calcium-Sensitive Dye Imaging of Activity in the Olfactory Bulb: Presynaptic Inhibition, Maps of Receptor Cell Input, and Oscillations
Gustatory System Development: New Experimental Approaches in Amphibian and Mammalian Embryos
Mammalian Taste Receptors
Researching Isolated Taste Receptor Cells: Deciphering Transduction Cascades with Patch-Clamp and Calcium: Imaging Techniques
Rapid Kinetic Measurements in Chemosensory Systems
Electrophysiological Recordings of Mammalian Taste

Higher-Order Studies in Taste and Olfaction
Activation of Neurons in the Trigeminal Subnucleus Caudalis (Vc) by Irritant Chemical Stimulation: Extracellular Single- Unit Recording and c-fos Immunohistochemical Methods
Methodological Considerations for Electrophysiological Recording and Analysis of Taste-Responsive Neurons in the Brainstem of the Rat
Electrophysiological Analysis of Olfactory Coding in the CNS
Electrophysiological Studies of Gustation in Awake Rats
Recording from Single Neurons in the Primary Taste Cortex of the Alert Macaque
Olfactory Learning and the Neurophysiological Study of Rat Prefrontal Function
Olfactory Event-Related Potentials
Application of Functional MRI in Olfactory Studies
Functional Imaging of Olfactory Activation in the Human Brain

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"…The authors are to be congratulated for doing step-by-step 'how-to' descriptions of many current techniques in the field, something any new researcher or student will find very useful…found the writing style to be consistently readable and informative. Much practical information is given…This book represents the kind of basic and well-written laboratory manual and overview textbook longed for in every field. I recommend it to graduate students, laboratory supervisors, and researchers…"
Perceptual Motor Skills, 2002