3rd Edition
Basic Laboratory Methods for Biotechnology Textbook and Laboratory Reference
Basic Laboratory Methods for Biotechnology, Third Edition is a versatile textbook that provides students with a solid foundation to pursue employment in the biotech industry and can later serve as a practical reference to ensure success at each stage in their career. The authors focus on basic principles and methods while skillfully including recent innovations and industry trends throughout.
Fundamental laboratory skills are emphasized, and boxed content provides step by step laboratory method instructions for ease of reference at any point in the students’ progress. Worked through examples and practice problems and solutions assist student comprehension. Coverage includes safety practices and instructions on using common laboratory instruments.
Key Features:
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Provides a valuable reference for laboratory professionals at all stages of their careers.
- Focuses on basic principles and methods to provide students with the knowledge needed to begin a career in the Biotechnology industry.
- Describes fundamental laboratory skills.
- Includes laboratory scenario-based questions that require students to write or discuss their answers to ensure they have mastered the chapter content.
- Updates reflect recent innovations and regulatory requirements to ensure students stay up to date.
- Tables, a detailed glossary, practice problems and solutions, case studies and anecdotes provide students with the tools needed to master the content.
UNIT I Biotechnology is the Transformation of Knowledge into Useful Products. 1. Techniques to Manipulate DNA: The Root of the Biotechnology Industry. 2. The Biotechnology Industry Branches Out. 3. The Business of Biotechnology: The Transformation of Knowledge into Products. UNIT II Introduction to Quality in Biotechnology Workplaces. Chapter 4. An Overview of Quality Principles in Biotechnology. 5. Quality in Research Laboratories. 6. Documentation: The Foundation of Quality. UNIT III Safety in the Laboratory. 7. Introduction to a Safe Workplace. 8. Working Safely in the Laboratory: General Considerations and Physical Hazards. 9. Working Safely with Chemicals. 10. Working Safely with Biological Materials. UNIT IV Math in the Biotechnology Laboratory: An Overview. 11. Basic Math Techniques. 12. Proportional Relationships. 13. Relationships and Graphing. 14. Descriptions of Data: Descriptive Statistics. UNIT V Obtaining Reproducible Laboratory Measurements. 16. Introduction to Instrumental Methods and Electricity. 17. The Measurement of Weight. 18. The Measurement of Volume. 19. The Measurement of Temperature. 20. The Measurement of pH, Selected Ions, and Conductivity. 21. Measurements Involving Light – Part A: Basic Principles and Instrumentation. UNIT VI Laboratory Solutions. 22. Preparation of Laboratory Solutions – Part A: Concentration Expressions and Calculations. 23. Preparation of Laboratory Solutions – Part B: Basic Procedures and Buffers. 24. Solutions: Associated Procedures and Information. 25. Laboratory Solutions to Support the Activity of Biological Macromolecules. UNIT VII Quality Assays and Tests. 26. Introduction to Quality Laboratory Tests and Assays. 27. Achieving Reproducible Results with Polymerase Chain Reaction Assays. 28. Measurements Involving Light – Part B: Assays. 29. Achieving Reproducible Immunoassay Results. UNIT VIII Cell Culture and Reproducibility. 30. Introduction to Quality Practices for Cell Culture. 31. Culture Media for Intact Cells. UNIT IX Basic Separation Methods. 32. Introduction to Filtration. 33. Introduction to Centrifugation. 34. Introduction to Bioseparations.UNIT X Biotechnology and Regulatory Affairs. 35. Biotechnology and the Regulation of Medical and Food Products. 36. The Lifecycles and Regulation of Pharmaceutical Products. 37. The Lifecycles and Regulation of Biopharmaceutical and Regenerative Medicine Products. 38. Quality Systems in a Regulated Production Facility. Appendix: Answers to Practice Problems. Acronyms. Glossary Terms.
UNIT III SAFETY IN THE LABORATORY
7 Introduction to a Safe Workplace
8 Working Safely in the Laboratory: General Considerations and Physical Hazards
9 Working Safely with Chemicals
10 Working Safely with Biological Materials
UNIT IV MATH IN THE BIOTECHNOLOGY LABORATORY: AN OVERVIEW
11 Basic Math Techniques
12 Proportional Relationships
13 Relationships and Graphing
14 Descriptions of Data: Descriptive Statistics
UNIT V LABORATORY MEASUREMENTS
15 Introduction to Quality Laboratory Measurements
16 Introduction to Instrumental Methods and Electricity
17 The Measurement of Weight
18 The Measurement of Volume
19 The Measurement of Temperature
20 The Measurement of pH, Selected Ions, and Conductivity
21 Measurements Involving Light A: Basic Principles and Instrumentation
UNIT VI LABORATORY SOLUTIONS
22 Preparation of Laboratory Solutions A: Concentration Expressions and Calculations
23 Preparation of Laboratory Solutions B: Basic Procedures and Buffers
24 Solutions: Associated Procedures and Information
25 Laboratory Solutions to Support the Activity of Biological Molecules
UNIT VII: QUALITY ASSAYS AND TESTS
26 Introduction to Quality Laboratory Assays Tests and Assays
27 Measurements Involving Light B: Assays
28 Immunoassays and Reproducibility
UNIT VIII CELL CULTURE AND REPRODUCIBILITY
29 Introduction to Quality Practices for Cell Culture
30 Culture Media for Intact Cells
UNIT IX BASIC SEPARATION METHODS
31 Introduction to Filtration
32 Introduction to Centrifugation (E2 32)
33 Introduction to Bioseparations (E2 33)
UNIT X BIOTECHNOLOGY AND REGULATORY AFFAIRS
34 Biotechnology and the Regulation of Medical and Food Products (E2 5)
35 The Lifecycles and Regulation of Pharmaceutical Products
36 The Lifecycles and Regulation of Biopharmaceutical and Regenerative Medicine Products
37 Quality Systems in a Regulated Production Facility
Biography
Lisa Seidman obtained her Ph.D. from the University of Wisconsin and has taught for more than thirty years in the Biotechnology Laboratory Technician Program at Madison Area Technical College. She is presently serving as Emeritus Faculty at the college.
Cynthia Moore received her Ph.D. in Microbiology from Temple University School of Medicine. She has taught for more than twenty years in the School of Biological Sciences at Illinois State University, where she currently serves as Emeritus Faculty.
Jeanette Mowery obtained her Ph.D. in Biomedical Science from the University of Texas Health Science Center-Houston. She has taught for more than 20 years in the Biotechnology Laboratory Technician Program at Madison Area Technical College, and is currently serving as Emeritus Faculty at the college.
